Hotspot

trnK-UUU-rps16

LSC · 4128-4715 · 588 bp

Back to Crocus

Pi 0.0193
MarkerSeek score 53.80
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
588
variable_sites
17
pi
0.0193
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT AAAAAGGCGCTCAACCTACA 50.0 45.0 58.2 58.0 702-711 bp 1.0 82.2
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT AAAAAGGCGCTCAACCTACA 50.0 45.0 58.2 58.0 701-710 bp 1.0 82.2
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT CTTGAAAAAGGCGCTCAACC 50.0 50.0 58.2 58.2 706-715 bp 1.0 80.5
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT CCTTGAAAAAGGCGCTCAAC 50.0 50.0 58.2 58.2 706-715 bp 1.0 80.5
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT CTTGAAAAAGGCGCTCAACC 50.0 50.0 58.2 58.2 705-714 bp 1.0 80.5