Hotspot

rbcL

LSC · 57620-59059 · 1440 bp

Back to Crinum

Pi 0.00324
MarkerSeek score 48.30
Primer available yes
Label rbcL

Feature Metadata

label_name
rbcL
feature_type
gene
region
LSC
length_bp
1440
variable_sites
7
pi
0.00324
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL_p1 TGAGTTGTAGGGAGGGACTT AAAAGGATTGGGCCGAGTTT 50.0 45.0 57.3 58.0 1553 bp 1.0 74.6
rbcL_p2 TGAGTTGTAGGGAGGGACTT TTAGGAAAAGGATTGGGCCG 50.0 50.0 57.3 57.9 1558 bp 1.0 73.5
rbcL_p3 TCATGAGTTGTAGGGAGGGA AAAAGGATTGGGCCGAGTTT 50.0 45.0 57.1 58.0 1556 bp 1.0 72.8
rbcL_p4 CATGAGTTGTAGGGAGGGAC AAAAGGATTGGGCCGAGTTT 55.0 45.0 57.0 58.0 1555 bp 1.0 72.4
rbcL_p5 TCATGAGTTGTAGGGAGGGA TTAGGAAAAGGATTGGGCCG 50.0 50.0 57.1 57.9 1561 bp 1.0 71.7