Hotspot

petA

LSC · 64592-65554 · 963 bp

Pi 0.00277

MarkerSeek score 60.50

Primer available yes

Label petA

Feature Metadata

label_name: petA
feature_type: gene
region: LSC
length_bp: 963
variable_sites: 4
pi: 0.00277
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA_p1	TTCAGTACAACGGCAGACTC	ATCGACAAGAATTCAGCGCT	50.0	45.0	57.6	58.0	1108 bp	1.0	76.7
petA_p2	TTCAGTACAACGGCAGACTC	TCGACAAGAATTCAGCGCTT	50.0	45.0	57.6	58.2	1107 bp	1.0	75.2
petA_p3	TTCAGTACAACGGCAGACTC	AACAAAGCAAGCAAAAGGGG	50.0	45.0	57.6	57.7	1157 bp	1.0	74.2
petA_p4	TTCAGTACAACGGCAGACTC	CAAAGCAAGCAAAAGGGGTT	50.0	45.0	57.6	57.7	1155 bp	1.0	74.2
petA_p5	TTCAGTACAACGGCAGACTC	GATCGACAAGAATTCAGCGC	50.0	50.0	57.6	57.6	1109 bp	1.0	73.9