Hotspot

rps15-ycf1

SSC · 127166-127548 · 383 bp

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Pi 0.0122
MarkerSeek score 62.50
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
383
variable_sites
7
pi
0.0122
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 AGTCTCCGTATCTTATCGGTGA ACCAATAACGGCGGTCATTT 45.5 45.0 57.9 58.2 552-557 bp 1.0 63.3
rps15-ycf1_p2 TGAAATTCAACAGATCCGCAG ACCAATAACGGCGGTCATTT 42.9 45.0 56.9 58.2 519-524 bp 1.0 63.1
rps15-ycf1_p3 GAAATTCAACAGATCCGCAGT ACCAATAACGGCGGTCATTT 42.9 45.0 56.9 58.2 518-523 bp 1.0 63.1
rps15-ycf1_p4 TCAACAGATCCGCAGTTTTT ACCAATAACGGCGGTCATTT 40.0 45.0 55.9 58.2 513-518 bp 1.0 63.1
rps15-ycf1_p5 TTCAACAGATCCGCAGTTTT ACCAATAACGGCGGTCATTT 40.0 45.0 55.9 58.2 514-519 bp 1.0 63.1