Hotspot

rpl32-trnL-UAG

SSC · 116619-117542 · 924 bp

Back to Crinum

Pi 0.00839
MarkerSeek score 66.00
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
924
variable_sites
10
pi
0.00839
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.90
markerseek_score
66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AGCAAAGTCAGTTTCTACCGG TCCTAAGAGCAGCGTGTCTA 47.6 50.0 58.2 57.9 865-1036 bp 1.0 74.0
rpl32-trnL-UAG_p2 GCAAAGTCAGTTTCTACCGGA TCCTAAGAGCAGCGTGTCTA 47.6 50.0 58.2 57.9 864-1035 bp 1.0 74.0
rpl32-trnL-UAG_p3 GCAAAGTCAGTTTCTACCGG TCCTAAGAGCAGCGTGTCTA 50.0 50.0 56.7 57.9 864-1035 bp 1.0 73.8
rpl32-trnL-UAG_p4 AGCAAAGTCAGTTTCTACCGG AGCAGCGTGTCTACCAATTT 47.6 45.0 58.2 57.8 858-1029 bp 1.0 73.5
rpl32-trnL-UAG_p5 GCAAAGTCAGTTTCTACCGGA AGCAGCGTGTCTACCAATTT 47.6 45.0 58.2 57.8 857-1028 bp 1.0 73.5