Hotspot

petA-psbJ

LSC · 65555-66699 · 1145 bp

Back to Crinum

Pi 0.00932
MarkerSeek score 60.20
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1145
variable_sites
16
pi
0.00932
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 CGTCCAAGGCCTTTTGTTTT GGTTCATATTCCGGGTTGGG 45.0 55.0 57.4 58.3 1287-1303 bp 1.0 73.7
petA-psbJ_p2 CGTCCAAGGCCTTTTGTTTT CATATTCCGGGTTGGGTTCA 45.0 50.0 57.4 57.6 1283-1299 bp 1.0 72.8
petA-psbJ_p3 CGTCCAAGGCCTTTTGTTTT GTTCATATTCCGGGTTGGGT 45.0 50.0 57.4 57.6 1286-1302 bp 1.0 72.8
petA-psbJ_p4 GATCCATTACGCGTCCAAGG GGTTCATATTCCGGGTTGGG 55.0 55.0 58.8 58.3 1298-1314 bp 1.0 72.2
petA-psbJ_p5 CGTCCAAGGCCTTTTGTTTT CGGTATTCCTGTGATCGGTT 45.0 50.0 57.4 57.4 1327-1343 bp 1.0 71.4