Hotspot

trnS-GCU-trnG-GCC

LSC · 8394-9548 · 1155 bp

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Pi 0.00317
MarkerSeek score 62.00
Primer available yes
Label trnS-GCU-trnG-GCC

Feature Metadata

label_name
trnS-GCU-trnG-GCC
feature_type
igs
region
LSC
length_bp
1155
variable_sites
5
pi
0.00317
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-GCC_p1 CAATCCGTCGCTTTAGTCCA CGGACGAATCACACTTTTACC 50.0 47.6 58.0 57.3 1216-1239 bp 1.0 67.4
trnS-GCU-trnG-GCC_p2 AATCCGTCGCTTTAGTCCAC CGGACGAATCACACTTTTACC 50.0 47.6 58.0 57.3 1215-1238 bp 1.0 67.4
trnS-GCU-trnG-GCC_p3 AGCAATCCGTCGCTTTAGTC CGGACGAATCACACTTTTACC 50.0 47.6 58.4 57.3 1218-1241 bp 1.0 64.6
trnS-GCU-trnG-GCC_p4 CGTCGCTTTAGTCCACTCAG CGGACGAATCACACTTTTACC 55.0 47.6 58.4 57.3 1211-1234 bp 1.0 64.4
trnS-GCU-trnG-GCC_p5 GCTTTAGTCCACTCAGCCAT CGGACGAATCACACTTTTACC 50.0 47.6 57.6 57.3 1207-1230 bp 1.0 64.2