Hotspot

trnK-UUU-rps16

LSC · 4063-4676 · 614 bp

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Pi 0.00546
MarkerSeek score 54.50
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
614
variable_sites
5
pi
0.00546
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AGTCTGGACGAATTCCTTGC AGGTGCTCAATCTACAGGAAC 50.0 47.6 57.9 57.4 773-777 bp 1.0 67.2
trnK-UUU-rps16_p2 AGTCTGGACGAATTCCTTGC GGTGCTCAATCTACAGGAACT 50.0 47.6 57.9 57.4 772-776 bp 1.0 67.2
trnK-UUU-rps16_p3 GTCTGGACGAATTCCTTGCT AGGTGCTCAATCTACAGGAAC 50.0 47.6 57.9 57.4 772-776 bp 1.0 67.2
trnK-UUU-rps16_p4 GTCTGGACGAATTCCTTGCT GGTGCTCAATCTACAGGAACT 50.0 47.6 57.9 57.4 771-775 bp 1.0 67.2
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT GGTGCTCAATCTACAGGAACT 50.0 47.6 58.2 57.4 715-719 bp 1.0 66.7