Hotspot

ndhG

SSC · 111930-112460 · 531 bp

Back to Crinipes

Pi 0.00377

MarkerSeek score 47.90

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 531
variable_sites: 2
pi: 0.00377
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	AAGAATTTTGACTCATTCTAAGT	AGTTTTAGTCAGTTCATGAAAAA	26.1	26.1	52.0	52.8	612 bp	1.0	40.3
ndhG_p2	AAGAATTTTGACTCATTCTAAGT	TCTTTTAGTTTTAGTCAGTTCATGA	26.1	28.0	52.0	54.7	618 bp	1.0	40.3
ndhG_p3	TGAAATTCTAAGAATTTTGACTCA	AGTTTTAGTCAGTTCATGAAAAA	25.0	26.1	52.9	52.8	621 bp	1.0	40.3
ndhG_p4	TGAAATTCTAAGAATTTTGACTCA	TCTTTTAGTTTTAGTCAGTTCATGA	25.0	28.0	52.9	54.7	627 bp	1.0	40.3
ndhG_p5	TCTAAGAATTTTGACTCATTCTAAGT	AGTTTTAGTCAGTTCATGAAAAA	26.9	26.1	54.7	52.8	615 bp	1.0	40.3