Hotspot

ndhF

SSC · 103971-106187 · 2217 bp

Back to Crinipes

Pi 0.00451

MarkerSeek score 49.00

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2217
variable_sites: 10
pi: 0.00451
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TGCATTTTGATGAGCCTCGT	TACGGTATGGGGGATAAGCC	45.0	55.0	58.2	58.4	2882-2916 bp	1.0	76.4
ndhF_p2	TTGCATTTTGATGAGCCTCG	TACGGTATGGGGGATAAGCC	45.0	55.0	57.1	58.4	2883-2917 bp	1.0	70.4
ndhF_p3	GCATTTTGATGAGCCTCGTT	TACGGTATGGGGGATAAGCC	45.0	55.0	57.1	58.4	2881-2915 bp	1.0	70.4
ndhF_p4	TGCATTTTGATGAGCCTCGT	AGGTTTGGTGAATCGGAAACA	45.0	42.9	58.2	58.1	2362 bp	0.5	50.1
ndhF_p5	GTTTGCTGGCTTATTTGGCA	AGGTTTGGTGAATCGGAAACA	45.0	42.9	57.5	58.1	2500 bp	0.5	47.9