Hotspot

rbcL-psaI

LSC · 58414-59258 · 845 bp

Back to Crinipes

Pi 0.0107

MarkerSeek score 64.90

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 845
variable_sites: 9
pi: 0.0107
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	TCGAGTTCCAACCGGTAGAT	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.2	58.0	947-956 bp	1.0	79.3
rbcL-psaI_p2	TCGAGTTCCAACCGGTAGAT	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.2	57.9	953-962 bp	1.0	78.7
rbcL-psaI_p3	AAATTCGAGTTCCAACCGGT	CCATTGCAATTGCCGGAAAT	45.0	45.0	57.7	58.0	951-960 bp	1.0	78.4
rbcL-psaI_p4	CGCAGCTTGTGAAGTATGGA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.3	58.0	979-988 bp	1.0	78.3
rbcL-psaI_p5	AAGCTTGCAAATGGAGTCCT	CCATTGCAATTGCCGGAAAT	45.0	45.0	57.7	58.0	1007-1016 bp	1.0	78.1