Hotspot

atpB-rbcL

LSC · 56197-56982 · 786 bp

Back to Crinipes

Pi 0.00386

MarkerSeek score 61.90

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 786
variable_sites: 3
pi: 0.00386
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TATCCAGCACGGGTCCAATA	ACTCCGGGGTGTAGTAAGTC	50.0	55.0	58.2	58.2	961-968 bp	1.0	78.0
atpB-rbcL_p2	TATCCAGCACGGGTCCAATA	CTCCGGGGTGTAGTAAGTCA	50.0	55.0	58.2	58.2	960-967 bp	1.0	78.0
atpB-rbcL_p3	CCAGCACGGGTCCAATAATT	ACTCCGGGGTGTAGTAAGTC	50.0	55.0	58.2	58.2	958-965 bp	1.0	77.8
atpB-rbcL_p4	CCAGCACGGGTCCAATAATT	CTCCGGGGTGTAGTAAGTCA	50.0	55.0	58.2	58.2	957-964 bp	1.0	77.8
atpB-rbcL_p5	CCGGGGGAAAAGTGATATCC	ACTCCGGGGTGTAGTAAGTC	55.0	55.0	57.7	58.2	976-983 bp	1.0	77.5