Hotspot

trnR-UCU-rps14

LSC · 38319-38703 · 385 bp

Back to Crinipes

Pi 0.0105

MarkerSeek score 61.00

Primer available yes

Label trnR-UCU-rps14

Feature Metadata

label_name: trnR-UCU-rps14
feature_type: igs
region: LSC
length_bp: 385
variable_sites: 4
pi: 0.0105
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-rps14_p1	AGAAGACCTCTGTCCTATCCA	ATGCATGTTTGTTACCGGGT	47.6	45.0	56.9	58.1	459-462 bp	1.0	64.1
trnR-UCU-rps14_p2	AGAAGACCTCTGTCCTATCCA	TCCGGACACATACTTCGAGA	47.6	50.0	56.9	57.9	487-490 bp	1.0	63.8
trnR-UCU-rps14_p3	AGGTTTAGAAGACCTCTGTCCT	ATGCATGTTTGTTACCGGGT	45.5	45.0	57.9	58.1	465-468 bp	1.0	63.5
trnR-UCU-rps14_p4	AGAAGACCTCTGTCCTATCCA	TTACCGGGTGCAACAAGATC	47.6	50.0	56.9	58.2	448-451 bp	1.0	63.4
trnR-UCU-rps14_p5	AGGTTTAGAAGACCTCTGTCCT	TCCGGACACATACTTCGAGA	45.5	50.0	57.9	57.9	493-496 bp	1.0	63.1