Hotspot

trnK-UUU-rps16

LSC · 3947-4500 · 554 bp

Back to Crinipes

Pi 0.0109

MarkerSeek score 63.70

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 554
variable_sites: 6
pi: 0.0109
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AGTCGCACTTAAAAGCCGAG	GGTGCTCAACCTACAAGAACT	50.0	47.6	58.6	57.9	685-688 bp	1.0	67.5
trnK-UUU-rps16_p2	TAGTCGCACTTAAAAGCCGA	GGTGCTCAACCTACAAGAACT	45.0	47.6	57.3	57.9	686-689 bp	1.0	66.2
trnK-UUU-rps16_p3	TCGCACTTAAAAGCCGAGTA	GGTGCTCAACCTACAAGAACT	45.0	47.6	57.3	57.9	683-686 bp	1.0	66.2
trnK-UUU-rps16_p4	CGCACTTAAAAGCCGAGTAC	GGTGCTCAACCTACAAGAACT	50.0	47.6	57.2	57.9	682-685 bp	1.0	65.8
trnK-UUU-rps16_p5	GTCGCACTTAAAAGCCGAGT	GGTGCTCAACCTACAAGAACT	50.0	47.6	58.9	57.9	684-687 bp	1.0	65.2