Hotspot

ycf1

SSC · 119194-124272 · 5079 bp

Pi 0.059

MarkerSeek score 78.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5079
variable_sites: 808
pi: 0.059
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AAGGAAGGCGTTTTGAGAGG	AAGAAATCCTCATTCCCCGC	50.0	50.0	58.1	57.9	600 bp	0.25	51.5
ycf1_p2	AAGGAAGGCGTTTTGAGAGG	GAAATCCTCATTCCCCGCTT	50.0	50.0	58.1	57.9	598 bp	0.25	51.5
ycf1_p3	AAGGAAGGCGTTTTGAGAGG	ACCTGATTTTCGTCGATCCG	50.0	50.0	58.1	58.1	678 bp	0.25	51.2
ycf1_p4	AAGGAAGGCGTTTTGAGAGG	CCTGATTTTCGTCGATCCGT	50.0	50.0	58.1	58.1	677 bp	0.25	51.2
ycf1_p5	AAGGAAGGCGTTTTGAGAGG	ATCAAAGGATCTATGCGGGC	50.0	50.0	58.1	57.8	656 bp	0.25	50.2