Hotspot

ndhF-rpl32

SSC · 107483-108588 · 1106 bp

Back to Crepis

Pi 0.0607
MarkerSeek score 85.60
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1106
variable_sites
182
pi
0.0607
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.95
markerseek_score
85.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GTTGCCGTCGGAAAAAGAAG ATATCTGCCCTTCCCGAGAA 50.0 50.0 58.0 57.9 1580-1664 bp 0.75 80.9
ndhF-rpl32_p2 TGCCGTCGGAAAAAGAAGAA ATATCTGCCCTTCCCGAGAA 45.0 50.0 58.0 57.9 1578-1662 bp 0.75 80.8
ndhF-rpl32_p3 TTGCCGTCGGAAAAAGAAGA ATATCTGCCCTTCCCGAGAA 45.0 50.0 58.0 57.9 1579-1663 bp 0.75 80.8
ndhF-rpl32_p4 GTTGCCGTCGGAAAAAGAAG TATCTGCCCTTCCCGAGAAA 50.0 50.0 58.0 58.1 1579-1663 bp 0.75 80.7
ndhF-rpl32_p5 TGGGTCGATCAAGTATCCGA ATATCTGCCCTTCCCGAGAA 50.0 50.0 57.9 57.9 1755-1839 bp 0.75 80.5