Hotspot

rbcL-accD

LSC · 55200-55708 · 509 bp

Back to Crepis

Pi 0.0593
MarkerSeek score 82.00
Primer available yes
Label rbcL-accD

Feature Metadata

label_name
rbcL-accD
feature_type
igs
region
LSC
length_bp
509
variable_sites
93
pi
0.0593
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.99
markerseek_score
82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-accD_p1 AGGCTACCAAATGGAGTCCT CAATAGGACCAAGACTGCCC 50.0 55.0 58.0 58.0 724-749 bp 0.5 68.8
rbcL-accD_p2 GGCTACCAAATGGAGTCCTG CAATAGGACCAAGACTGCCC 55.0 55.0 58.0 58.0 723-748 bp 0.5 68.7
rbcL-accD_p3 CTAACAGGGGGATTCACTGC CAATAGGACCAAGACTGCCC 55.0 55.0 58.0 58.0 1256-1281 bp 0.5 66.5
rbcL-accD_p4 TTGAATGCTACTGCGGGTAC CAATAGGACCAAGACTGCCC 50.0 55.0 58.0 58.0 1346-1371 bp 0.5 66.2
rbcL-accD_p5 AATGCTACTGCGGGTACATG CAATAGGACCAAGACTGCCC 50.0 55.0 58.0 58.0 1343-1368 bp 0.5 66.0