Hotspot

trnF-GAA-ndhJ

LSC · 46696-47321 · 626 bp

Back to Crepis

Pi 0.0571
MarkerSeek score 85.60
Primer available yes
Label trnF-GAA-ndhJ

Feature Metadata

label_name
trnF-GAA-ndhJ
feature_type
igs
region
LSC
length_bp
626
variable_sites
99
pi
0.0571
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.99
markerseek_score
85.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-GAA-ndhJ_p1 CCTCGTGTCACCAGTTCAAA TGGCATTCCATTGCTGTCAT 50.0 45.0 58.1 58.1 1011-1051 bp 1.0 89.0
trnF-GAA-ndhJ_p2 CCTCGTGTCACCAGTTCAAA CCCAATGCGCCTATGATGTA 50.0 50.0 58.1 57.8 959-993 bp 0.875 83.8
trnF-GAA-ndhJ_p3 CCTCGTGTCACCAGTTCAAA ATAAAAAGTCGCGGCTCACT 50.0 45.0 58.1 57.9 1206-1231 bp 0.5 63.8
trnF-GAA-ndhJ_p4 AGAGACTCGATGGAAGCTGT ATAAAAAGTCGCGGCTCACT 50.0 45.0 58.2 57.9 1919-1942 bp 0.5 63.3
trnF-GAA-ndhJ_p5 CCTCGTGTCACCAGTTCAAA ATTATCTCCGCTCCCAATGC 50.0 50.0 58.1 57.8 993 bp 0.125 43.1