Hotspot

trnT-UGU-trnL-UAA

LSC · 45190-45756 · 567 bp

Back to Crepis

Pi 0.0705
MarkerSeek score 88.60
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
567
variable_sites
111
pi
0.0705
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.99
markerseek_score
88.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 GCGATGCTCTAACCTCTGAG CCGTAGCGTCTACCGATTTC 55.0 55.0 57.9 58.2 602-653 bp 1.0 89.0
trnT-UGU-trnL-UAA_p2 GGAATCGAACCGATGACCAT CCGTAGCGTCTACCGATTTC 50.0 55.0 57.8 58.2 634-685 bp 1.0 88.1
trnT-UGU-trnL-UAA_p3 GCGATGCTCTAACCTCTGAG CGTCTACCGATTTCGCCATA 55.0 50.0 57.9 57.6 596-647 bp 1.0 87.7
trnT-UGU-trnL-UAA_p4 GGAATCGAACCGATGACCAT CGTCTACCGATTTCGCCATA 50.0 50.0 57.8 57.6 628-679 bp 1.0 86.8
trnT-UGU-trnL-UAA_p5 GCGATGCTCTAACCTCTGAG CGTAGCGTCTACCGATTTCG 55.0 55.0 57.9 58.6 601-652 bp 1.0 86.0