Hotspot

ycf3-trnS-GGA

LSC · 42842-43757 · 916 bp

Back to Crepis

Pi 0.0498
MarkerSeek score 83.70
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
916
variable_sites
128
pi
0.0498
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.99
markerseek_score
83.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 AACATAGCATCTGAGGCTGC TTGGGTATGGCTCCGACTAT 50.0 50.0 58.0 57.9 1830-1953 bp 1.0 90.8
ycf3-trnS-GGA_p2 AACATAGCATCTGAGGCTGC TGGGTATGGCTCCGACTATT 50.0 50.0 58.0 57.9 1829-1952 bp 1.0 90.7
ycf3-trnS-GGA_p3 TCAACGCTTCCCAATTTCCA TTGGGTATGGCTCCGACTAT 45.0 50.0 58.0 57.9 2108-2231 bp 0.875 84.9
ycf3-trnS-GGA_p4 TCAACGCTTCCCAATTTCCA TGGGTATGGCTCCGACTATT 45.0 50.0 58.0 57.9 2107-2230 bp 0.875 84.8
ycf3-trnS-GGA_p5 TCAACGCTTCCCAATTTCCA TGAAATGCGCCTGGATAACA 45.0 45.0 58.0 57.9 2139-2262 bp 0.875 84.6