Hotspot

trnT-GGU-psbD

LSC · 30213-31218 · 1006 bp

Back to Crepis

Pi 0.0452
MarkerSeek score 82.00
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1006
variable_sites
118
pi
0.0452
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.98
markerseek_score
82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GAATCAAACCGAGAGACCCC GAAACGGTCCCTACGTAACC 55.0 55.0 58.0 58.0 1374-1600 bp 1.0 91.8
trnT-GGU-psbD_p2 GAATCAAACCGAGAGACCCC TGGACCAGAGAATGCGATTG 55.0 50.0 58.0 58.0 1740-1966 bp 1.0 90.8
trnT-GGU-psbD_p3 GAATCAAACCGAGAGACCCC AAACTAGGCGCAAAGAACCA 55.0 45.0 58.0 58.0 1811-2037 bp 1.0 90.7
trnT-GGU-psbD_p4 GAATCAAACCGAGAGACCCC AACTAGGCGCAAAGAACCAA 55.0 45.0 58.0 58.0 1810-2036 bp 1.0 90.7
trnT-GGU-psbD_p5 GAATCAAACCGAGAGACCCC ACCAAAACTAGGCGCAAAGA 55.0 45.0 58.0 58.0 1815-2041 bp 1.0 90.7