Hotspot

psbM-trnD-GUC

LSC · 10913-11513 · 601 bp

Back to Crepis

Pi 0.0462
MarkerSeek score 84.40
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
601
variable_sites
78
pi
0.0462
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.95
markerseek_score
84.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 AAATGCAAGAATATTTACTTCCA CTGTCAAGGCGGACGTTG 26.1 61.1 52.7 58.8 654-750 bp 1.0 52.0
psbM-trnD-GUC_p2 AATGCAAGAATATTTACTTCCAT CTGTCAAGGCGGACGTTG 26.1 61.1 52.4 58.8 653-749 bp 1.0 52.0
psbM-trnD-GUC_p3 AAATGCAAGAATATTTACTTCCAT CTGTCAAGGCGGACGTTG 25.0 61.1 53.3 58.8 654-750 bp 1.0 52.0
psbM-trnD-GUC_p4 TAAATGCAAGAATATTTACTTCCA CTGTCAAGGCGGACGTTG 25.0 61.1 52.7 58.8 655-751 bp 1.0 52.0
psbM-trnD-GUC_p5 AAATGCAAGAATATTTACTTCCA CCTGTCAAGGCGGACGTT 26.1 61.1 52.7 60.0 655-751 bp 1.0 52.0