Hotspot

trnC-GCA-petN

LSC · 9435-10221 · 787 bp

Back to Crepis

Pi 0.0575
MarkerSeek score 84.60
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
787
variable_sites
130
pi
0.0575
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.97
markerseek_score
84.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 TTTCCCCAGTTCAAATCCGG AGCCCAAGCGAGACTTACTA 50.0 50.0 58.1 58.2 853-915 bp 0.875 84.8
trnC-GCA-petN_p2 GGGGGACTGCAAATCCTTTT AGCCCAAGCGAGACTTACTA 50.0 50.0 58.4 58.2 871-933 bp 0.875 82.5
trnC-GCA-petN_p3 TTTCCCCAGTTCAAATCCGG ATCAACGGGCTATAATGGGC 50.0 50.0 58.1 57.8 1309-1379 bp 0.5 63.1
trnC-GCA-petN_p4 GGGGGACTGCAAATCCTTTT ATCAACGGGCTATAATGGGC 50.0 50.0 58.4 57.8 1327-1397 bp 0.5 60.9
trnC-GCA-petN_p5 TTTCCCCAGTTCAAATCCGG GGCTATAATGGGCGGTAGTC 50.0 55.0 58.1 57.6 1302-1372 bp 0.375 55.5