Hotspot

trnS-GCU-trnC-GCA

LSC · 8603-9353 · 751 bp

Back to Crepis

Pi 0.0432
MarkerSeek score 82.90
Primer available yes
Label trnS-GCU-trnC-GCA

Feature Metadata

label_name
trnS-GCU-trnC-GCA
feature_type
igs
region
LSC
length_bp
751
variable_sites
98
pi
0.0432
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnC-GCA_p1 CTTTCGTCCACTCAGCCATC CCGGATTTGAACTGGGGAAA 55.0 50.0 58.6 58.1 831-849 bp 1.0 84.2
trnS-GCU-trnC-GCA_p2 CTTTCGTCCACTCAGCCATC AAAAGGATTTGCAGTCCCCC 55.0 50.0 58.6 58.4 813-831 bp 1.0 82.0
trnS-GCU-trnC-GCA_p3 TTTCGTCCACTCAGCCATCT CCGGATTTGAACTGGGGAAA 50.0 50.0 59.0 58.1 830-848 bp 1.0 81.1
trnS-GCU-trnC-GCA_p4 TCCACTCAGCCATCTCTCC CCGGATTTGAACTGGGGAAA 57.9 50.0 58.1 58.1 825-843 bp 1.0 80.5
trnS-GCU-trnC-GCA_p5 ATTAGCAATCCGCCGCTTT CCGGATTTGAACTGGGGAAA 47.4 50.0 58.2 58.1 846-864 bp 1.0 79.6