Hotspot

trnR-ACG-trnN-GUU

Genome · 107367-107800 · 434 bp

Back to Crepidomanes

Pi 0.186

MarkerSeek score 89.90

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: Genome
length_bp: 434
variable_sites: 77
pi: 0.186
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 89.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	GGATGGAGAGAGGAAGGGTT	CTCGGTAGCTCAGTGGTAGA	55.0	55.0	58.1	58.0	624-763 bp	1.0	89.0
trnR-ACG-trnN-GUU_p2	GGATGGAGAGAGGAAGGGTT	TCGGTAGCTCAGTGGTAGAG	55.0	55.0	58.1	58.0	623-762 bp	1.0	89.0
trnR-ACG-trnN-GUU_p3	GGATGGAGAGAGGAAGGGTT	GCAATCTTCCTCGGTAGCTC	55.0	55.0	58.1	58.1	633-772 bp	1.0	88.1
trnR-ACG-trnN-GUU_p4	GAACAGATCCAGTGGAGACG	CTCGGTAGCTCAGTGGTAGA	55.0	55.0	57.7	58.0	598-697 bp	1.0	87.7
trnR-ACG-trnN-GUU_p5	GAACAGATCCAGTGGAGACG	TCGGTAGCTCAGTGGTAGAG	55.0	55.0	57.7	58.0	597-696 bp	1.0	87.7