Hotspot

trnS-UGA-psbC

Genome · 40924-41676 · 753 bp

Back to Crepidomanes

Pi 0.242

MarkerSeek score 82.50

Primer available yes

Label trnS-UGA-psbC

Feature Metadata

label_name: trnS-UGA-psbC
feature_type: igs
region: Genome
length_bp: 753
variable_sites: 176
pi: 0.242
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 82.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbC_p1	GGAGAGGCAAGTACAACAGG	GATCTTCGTGCTCCATGGTT	55.0	50.0	57.9	58.0	1627-1653 bp	1.0	88.9
trnS-UGA-psbC_p2	GGAGAGGCAAGTACAACAGG	CAGTAGGGGGAGTAGCTACC	55.0	60.0	57.9	58.0	1488 bp	0.5	59.2
trnS-UGA-psbC_p3	GGAGAGGCAAGTACAACAGG	CATTTGTGGCATGCAGGAAG	55.0	50.0	57.9	58.0	1384 bp	0.5	59.2
trnS-UGA-psbC_p4	TGCTGATTCAACCTTAGGGC	CAGTAGGGGGAGTAGCTACC	50.0	60.0	57.9	58.0	1685 bp	0.5	59.0
trnS-UGA-psbC_p5	TGCTGATTCAACCTTAGGGC	CATTTGTGGCATGCAGGAAG	50.0	50.0	57.9	58.0	1581 bp	0.5	58.9