Hotspot

psbM-trnD-GUC

Genome · 38238-38689 · 452 bp

Back to Crepidomanes

Pi 0.219
MarkerSeek score 83.50
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
Genome
length_bp
452
variable_sites
96
pi
0.219
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
83.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 AGTGCAGTAGCTATGAATGCA GCGCTCTAACCGATTGAACT 42.9 50.0 57.5 58.4 518-719 bp 1.0 74.6
psbM-trnD-GUC_p2 GTGCAGTAGCTATGAATGCA GCGCTCTAACCGATTGAACT 45.0 50.0 55.9 58.4 517-718 bp 1.0 70.3
psbM-trnD-GUC_p3 TGCAGTAGCTATGAATGCAAGA GCGCTCTAACCGATTGAACT 40.9 50.0 57.9 58.4 516-717 bp 1.0 69.8
psbM-trnD-GUC_p4 GAGTGCAGTAGCTATGAATGC GCGCTCTAACCGATTGAACT 47.6 50.0 56.9 58.4 519-720 bp 1.0 69.8
psbM-trnD-GUC_p5 AGTGCAGTAGCTATGAATGCA GGATTGACGGGTCTCGAAC 42.9 57.9 57.5 58.0 562-763 bp 1.0 68.9