Hotspot

psbA-matK

Genome · 7108-7708 · 601 bp

Back to Crepidomanes

Pi 0.246
MarkerSeek score 83.80
Primer available yes
Label psbA-matK

Feature Metadata

label_name
psbA-matK
feature_type
igs
region
Genome
length_bp
601
variable_sites
140
pi
0.246
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
83.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA-matK_p1 TGCTCAGCCTGGAAAACAAT TTCAAGCCCCAATCATGCTT 45.0 45.0 58.0 58.1 1586-1599 bp 1.0 89.2
psbA-matK_p2 TGCTCAGCCTGGAAAACAAT TTTCAAGCCCCAATCATGCT 45.0 45.0 58.0 58.1 1587-1600 bp 1.0 89.2
psbA-matK_p3 GTGCTAGTGATCCAGTTGCA TTCAAGCCCCAATCATGCTT 50.0 45.0 57.9 58.1 1088-1101 bp 1.0 89.1
psbA-matK_p4 AGAGGTTGCGGTTAAAAGGG TTCAAGCCCCAATCATGCTT 50.0 45.0 58.1 58.1 1155-1168 bp 1.0 89.0
psbA-matK_p5 AGAGGTTGCGGTTAAAAGGG TTTCAAGCCCCAATCATGCT 50.0 45.0 58.1 58.1 1156-1169 bp 1.0 89.0