Hotspot

trnH-GUG-psbA

Genome · 5695-6045 · 351 bp

Back to Crepidomanes

Pi 0.317

MarkerSeek score 79.20

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: Genome
length_bp: 351
variable_sites: 91
pi: 0.317
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	TTGCCTTAATCCACTTGGCT	TGAAGCTCCTTCTGTAAACGG	45.0	47.6	57.4	58.0	358-404 bp	1.0	78.9
trnH-GUG-psbA_p2	TTGCCTTAATCCACTTGGCT	GAAGCTCCTTCTGTAAACGGA	45.0	47.6	57.4	57.7	357-403 bp	1.0	76.7
trnH-GUG-psbA_p3	CGCGTAATGGATCCACAATC	TGAAGCTCCTTCTGTAAACGG	50.0	47.6	57.1	58.0	380-426 bp	1.0	76.1
trnH-GUG-psbA_p4	TAATCCACTTGGCTACGTCC	TGAAGCTCCTTCTGTAAACGG	50.0	47.6	57.0	58.0	352-398 bp	1.0	75.9
trnH-GUG-psbA_p5	TGCCTTAATCCACTTGGCTAC	TGAAGCTCCTTCTGTAAACGG	47.6	47.6	58.0	58.0	357-403 bp	1.0	75.7