Hotspot

psaC-ndhE

SSC · 147792-148281 · 490 bp

Back to Crepidium

Pi 0.0122

MarkerSeek score 63.50

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 490
variable_sites: 6
pi: 0.0122
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	AGCTCGAACACATTGGGTAC	TATAGCTATTGCGGCTGCTG	50.0	50.0	57.9	57.9	659-666 bp	1.0	79.3
psaC-ndhE_p2	ACATCCGTCCCAAGGTATCA	TATAGCTATTGCGGCTGCTG	50.0	50.0	58.1	57.9	701-708 bp	1.0	79.0
psaC-ndhE_p3	GCTCGAACACATTGGGTACA	TATAGCTATTGCGGCTGCTG	50.0	50.0	58.2	57.9	658-665 bp	1.0	78.5
psaC-ndhE_p4	AGCTTTACATCCGTCCCAAG	TATAGCTATTGCGGCTGCTG	50.0	50.0	57.6	57.9	707-714 bp	1.0	76.8
psaC-ndhE_p5	TTTCCAATACATCCGTGGGG	TATAGCTATTGCGGCTGCTG	50.0	50.0	57.6	57.9	681-688 bp	1.0	76.6