Hotspot

ndhF-rpl32

SSC · 142763-143668 · 906 bp

Back to Crepidium

Pi 0.0188
MarkerSeek score 69.70
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
906
variable_sites
17
pi
0.0188
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGAAGCGGAAAAAGGGGTAT GCTTTTACTGCCTCTAAAGATCC 50.0 43.5 57.9 57.3 1048-1105 bp 1.0 50.3
ndhF-rpl32_p2 GGAAGCGGAAAAAGGGGTAT AGAAAAAGCTTTTACTGCCTCT 50.0 36.4 57.9 56.2 1055-1112 bp 1.0 49.7
ndhF-rpl32_p3 GGAAGCGGAAAAAGGGGTAT AAGAAAAAGCTTTTACTGCCTCT 50.0 34.8 57.9 56.9 1056-1113 bp 1.0 47.3
ndhF-rpl32_p4 AACTGGAAGCGGAAAAAGGG GCTTTTACTGCCTCTAAAGATCC 50.0 43.5 58.7 57.3 1052-1109 bp 1.0 46.0
ndhF-rpl32_p5 GGAAGCGGAAAAAGGGGTAT GAAAAAGCTTTTACTGCCTCT 50.0 38.1 57.9 54.7 1054-1111 bp 1.0 45.6