Hotspot

ycf2-trnL-CAA

IRa · 123334-124298 · 965 bp

Back to Crepidium

Pi 0.00319

MarkerSeek score 63.20

Primer available yes

Label ycf2-trnL-CAA

Feature Metadata

label_name: ycf2-trnL-CAA
feature_type: igs
region: IRa
length_bp: 965
variable_sites: 3
pi: 0.00319
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-trnL-CAA_p1	AAAGAGATGGCTTTTCCCGG	AAATGGTAGACACGCGAGAC	50.0	50.0	58.2	58.0	1082-1108 bp	1.0	80.8
ycf2-trnL-CAA_p2	AAAGAGATGGCTTTTCCCGG	TGAAATGGTAGACACGCGAG	50.0	50.0	58.2	58.0	1084-1110 bp	1.0	80.8
ycf2-trnL-CAA_p3	ATGGCTTTTCCCGGATGAAA	AAATGGTAGACACGCGAGAC	45.0	50.0	57.8	58.0	1076-1102 bp	1.0	80.2
ycf2-trnL-CAA_p4	TGGCTTTTCCCGGATGAAAT	AAATGGTAGACACGCGAGAC	45.0	50.0	57.8	58.0	1075-1101 bp	1.0	80.2
ycf2-trnL-CAA_p5	TGGCTTTTCCCGGATGAAAT	TGAAATGGTAGACACGCGAG	45.0	50.0	57.8	58.0	1077-1103 bp	1.0	80.2