Hotspot

clpP1-psbB

LSC · 100772-101540 · 769 bp

Back to Crepidium

Pi 0.0184

MarkerSeek score 57.10

Primer available yes

Label clpP1-psbB

Feature Metadata

label_name: clpP1-psbB
feature_type: igs
region: LSC
length_bp: 769
variable_sites: 14
pi: 0.0184
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP1-psbB_p1	CTTTTGGAACACCAATGGGC	CACGATACCAAGGTAAACCCA	50.0	47.6	57.8	57.7	813-817 bp	1.0	70.0
clpP1-psbB_p2	CTTTTGGAACACCAATGGGC	ACACGATACCAAGGTAAACCC	50.0	47.6	57.8	57.7	814-818 bp	1.0	70.0
clpP1-psbB_p3	TCCTCTCCAGGAATCCGAAA	CACGATACCAAGGTAAACCCA	50.0	47.6	57.8	57.7	838-842 bp	1.0	69.3
clpP1-psbB_p4	TCCTCTCCAGGAATCCGAAA	ACACGATACCAAGGTAAACCC	50.0	47.6	57.8	57.7	839-843 bp	1.0	69.3
clpP1-psbB_p5	TTTGGAACACCAATGGGCAT	CACGATACCAAGGTAAACCCA	45.0	47.6	58.3	57.7	811-815 bp	1.0	69.2