Hotspot

rpl20-rps12

LSC · 97510-98289 · 780 bp

Back to Crepidium

Pi 0.00781

MarkerSeek score 64.70

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 780
variable_sites: 6
pi: 0.00781
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TCCGAGCTGTATATCCTCGT	GAAATCTCCCGCTCTTCGAG	50.0	55.0	57.4	58.2	860-872 bp	1.0	75.9
rpl20-rps12_p2	TCCGAGCTGTATATCCTCGT	TAGAAACGCAAGACAGCCAA	50.0	45.0	57.4	57.8	893-905 bp	1.0	75.1
rpl20-rps12_p3	TCCGAGCTGTATATCCTCGT	TTAGAAACGCAAGACAGCCA	50.0	45.0	57.4	57.8	894-906 bp	1.0	75.1
rpl20-rps12_p4	TCCGAGCTGTATATCCTCGT	TGTTACGAAATCTCCCGCTC	50.0	50.0	57.4	57.7	866-878 bp	1.0	74.8
rpl20-rps12_p5	CGACGTCTCCGAGCTGTATA	GAAATCTCCCGCTCTTCGAG	55.0	55.0	58.8	58.2	867-879 bp	1.0	74.1