Hotspot

trnD-GUC-trnY-GUA

LSC · 57978-58290 · 313 bp

Back to Crepidium

Pi 0.0256

MarkerSeek score 60.40

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 313
variable_sites: 8
pi: 0.0256
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	ATTCATCGGATCGGGACTGA	CGAGATGTCTACGCTGGTTC	50.0	55.0	58.3	58.2	435-455 bp	1.0	77.7
trnD-GUC-trnY-GUA_p2	CGGTGCTCTGACCAATTGAA	CGAGATGTCTACGCTGGTTC	50.0	55.0	58.5	58.2	380-400 bp	1.0	76.5
trnD-GUC-trnY-GUA_p3	ATTCATCGGATCGGGACTGA	GGGGACGGACTGTAAATTCG	50.0	55.0	58.3	58.4	459-479 bp	1.0	76.2
trnD-GUC-trnY-GUA_p4	ATTCATCGGATCGGGACTGA	GGGACGGACTGTAAATTCGT	50.0	50.0	58.3	57.6	458-478 bp	1.0	76.0
trnD-GUC-trnY-GUA_p5	TCAATTCATCGGATCGGGAC	CGAGATGTCTACGCTGGTTC	50.0	55.0	57.5	58.2	438-458 bp	1.0	75.8