Hotspot

rpoC1-rpoB

LSC · 50416-50420 · 5 bp

Back to Crepidium

Pi 0.2

MarkerSeek score 87.50

Primer available yes

Label rpoC1-rpoB

Feature Metadata

label_name: rpoC1-rpoB
feature_type: igs
region: LSC
length_bp: 5
variable_sites: 1
pi: 0.2
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 87.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1-rpoB_p1	GGGAAACTGGTCCAATTCGA	AGAGGGTGCTCCAGAATCTT	50.0	50.0	57.8	58.0	193 bp	1.0	78.6
rpoC1-rpoB_p2	GGGAAACTGGTCCAATTCGA	TTGCTCGTTCGAGAACTACG	50.0	50.0	57.8	58.0	168 bp	1.0	78.6
rpoC1-rpoB_p3	GGGGGAAACTGGTCCAATTC	AGAGGGTGCTCCAGAATCTT	55.0	50.0	58.4	58.0	195 bp	1.0	76.6
rpoC1-rpoB_p4	GGGGGAAACTGGTCCAATTC	TTGCTCGTTCGAGAACTACG	55.0	50.0	58.4	58.0	170 bp	1.0	76.6
rpoC1-rpoB_p5	GGGAAACTGGTCCAATTCGA	TTCGATTGCTCGTTCGAGAA	50.0	45.0	57.8	57.7	173 bp	1.0	76.2