Hotspot

ccsA-ndhD

SSC · 114703-114980 · 278 bp

Back to Crepidiastrum

Pi 0.0489

MarkerSeek score 70.60

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 278
variable_sites: 13
pi: 0.0489
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	CGGCTCTTCTTTGTGGATCA	GTTTTTGATTCTGGACCGCG	50.0	50.0	57.9	58.0	937-949 bp	1.0	81.9
ccsA-ndhD_p2	CGGCTCTTCTTTGTGGATCA	CTCCCGGCCAACGTAATTTA	50.0	50.0	57.9	58.0	1413-1425 bp	1.0	81.1
ccsA-ndhD_p3	TTTTTACCCAGGGCTTTGCT	CTCCCGGCCAACGTAATTTA	45.0	50.0	57.9	58.0	1548 bp	0.5	59.0
ccsA-ndhD_p4	TTTTTACCCAGGGCTTTGCT	GTTTTTGATTCTGGACCGCG	45.0	50.0	57.9	58.0	1072 bp	0.5	58.9
ccsA-ndhD_p5	TTTTTACCCAGGGCTTTGCT	GCTTGCATTACCGGGTATGA	45.0	50.0	57.9	58.0	1277 bp	0.5	58.9