Hotspot

petD-rpoA

LSC · 77503-77699 · 197 bp

Back to Crepidiastrum

Pi 0.0558

MarkerSeek score 68.30

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 197
variable_sites: 11
pi: 0.0558
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	GTTTCAGTACCTGCGGGATT	AACACACGGTTCCTTTACCC	50.0	50.0	57.9	58.0	664 bp	1.0	81.1
petD-rpoA_p2	CTCGAGCCGGATGATGAAAA	AACACACGGTTCCTTTACCC	50.0	50.0	58.1	58.0	1035 bp	1.0	80.8
petD-rpoA_p3	CTCGAGCCGGATGATGAAAA	TGAAGCGTCCATTTGTGTCA	50.0	45.0	58.1	58.0	1445 bp	1.0	80.6
petD-rpoA_p4	CTCGAGCCGGATGATGAAAA	ATTGCCTCAAACGGTCCAAT	50.0	45.0	58.1	58.1	909 bp	1.0	80.5
petD-rpoA_p5	GTTTCAGTACCTGCGGGATT	TGAAGCGTCCATTTGTGTCA	50.0	45.0	57.9	58.0	1074 bp	1.0	80.5