Hotspot

atpB-rbcL

LSC · 54088-54859 · 772 bp

Back to Crepidiastrum

Pi 0.0195

MarkerSeek score 70.20

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 772
variable_sites: 15
pi: 0.0195
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CTGGCGGAAAAGCTACATCT	CACCAGCTTTGAATCCAACAC	50.0	47.6	58.0	58.0	933-943 bp	1.0	73.1
atpB-rbcL_p2	CTGGCGGAAAAGCTACATCT	ACCAGCTTTGAATCCAACACT	50.0	42.9	58.0	58.1	932-942 bp	1.0	72.8
atpB-rbcL_p3	CGCCCCAGTTTCTTTTTGTC	CACCAGCTTTGAATCCAACAC	50.0	47.6	57.9	58.0	886-896 bp	1.0	72.3
atpB-rbcL_p4	CGCCCCAGTTTCTTTTTGTC	ACCAGCTTTGAATCCAACACT	50.0	42.9	57.9	58.1	885-895 bp	1.0	72.1
atpB-rbcL_p5	TTCTTTTTGTCAAGCGTGGC	CACCAGCTTTGAATCCAACAC	45.0	47.6	57.8	58.0	877-887 bp	1.0	71.8