primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	CCGTGCTAACCTTGGTATGG	20	20	55.0	55.0	58.1	58.3	0.0	0.0	0.465225	78	481	522-533	1.000000	83.2
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.469587	78	481	536-547	1.000000	83.1
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTGCCTTGATCCAC	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.471094	78	481	535-546	1.000000	83.0
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.471094	78	481	537-548	1.000000	83.0
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	GATCCACTTGGCTACATCCG	ACCGTGCTAACCTTGGTATG	20	20	55.0	50.0	58.1	57.6	0.0	0.0	0.525510	78	481	523-534	1.000000	82.7
trnS-GCU-trnC-GCA_p1	trnS-GCU-trnC-GCA	1	ACGGAAAGAGAGGGATTCGA	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	58.2	58.1	0.0	0.0	0.233476	8660	9414	904-906	1.000000	80.4
trnS-GCU-trnC-GCA_p2	trnS-GCU-trnC-GCA	2	ACGGAAAGAGAGGGATTCGA	AAAAGGATTTGCAGTCCCCC	20	20	50.0	50.0	58.2	58.4	0.0	14.6	0.506839	8660	9414	886-888	1.000000	78.3
trnS-GCU-trnC-GCA_p3	trnS-GCU-trnC-GCA	3	CTTTCGTCCACTCAGCCATC	CCGGATTTGAACTGGGGAAA	20	20	55.0	50.0	58.6	58.1	0.0	0.0	0.718384	8660	9414	840-842	1.000000	76.7
trnS-GCU-trnC-GCA_p4	trnS-GCU-trnC-GCA	4	AACGGAAAGAGAGGGATTCG	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	57.3	58.1	0.0	0.0	0.756923	8660	9414	905-907	1.000000	76.2
trnS-GCU-trnC-GCA_p5	trnS-GCU-trnC-GCA	5	CTTTCGTCCACTCAGCCATC	AAAAGGATTTGCAGTCCCCC	20	20	55.0	50.0	58.6	58.4	0.0	14.6	0.991747	8660	9414	822-824	1.000000	74.6
petN-psbM_p1	petN-psbM	1	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTT	20	20	50.0	40.0	58.2	55.6	0.0	0.0	2.573941	9959	10450	647-654	1.000000	62.6
petN-psbM_p2	petN-psbM	2	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTTT	20	21	50.0	38.1	58.2	56.4	0.0	0.0	2.785752	9959	10450	647-654	1.000000	60.9
petN-psbM_p3	petN-psbM	3	ATAGTAAGTCTCGCTTGGGC	TGCTACTGCACTGTTCATTT	20	20	50.0	40.0	57.1	55.6	0.0	0.0	3.315377	9959	10450	648-655	1.000000	56.7
petN-psbM_p4	petN-psbM	4	CTCGCTTGGGCTGCTTTAAT	TGCTACTGCACTGTTCATTT	20	20	50.0	40.0	58.9	55.6	0.0	0.0	3.324543	9959	10450	639-646	1.000000	56.6
petN-psbM_p5	petN-psbM	5	TGGGCTGCTTTAATGGTAGTC	TGCTACTGCACTGTTCATTT	21	20	47.6	40.0	58.0	55.6	0.0	0.0	3.423261	9959	10450	633-640	1.000000	55.9
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	GGAACTAAAATGAACAGTGCAGT	TTCAATAGGCAAGAGCACCG	23	20	39.1	50.0	57.6	58.3	0.0	0.0	3.700923	10556	11208	770-775	1.000000	52.9
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	AAAATGAACAGTGCAGTAGC	TTCAATAGGCAAGAGCACCG	20	20	40.0	50.0	54.5	58.3	0.0	0.0	3.756773	10556	11208	764-769	1.000000	52.5
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	ACTAAAATGAACAGTGCAGTAGC	TTCAATAGGCAAGAGCACCG	23	20	39.1	50.0	57.4	58.3	0.0	0.0	3.875520	10556	11208	767-772	1.000000	51.5
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	AGGAACTAAAATGAACAGTGCAG	TTCAATAGGCAAGAGCACCG	23	20	39.1	50.0	57.3	58.3	0.0	0.0	3.958013	10556	11208	771-776	1.000000	50.8
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	AGGAACTAAAATGAACAGTGCA	TTCAATAGGCAAGAGCACCG	22	20	36.4	50.0	56.2	58.3	0.0	0.0	4.069095	10556	11208	771-776	1.000000	49.9
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	20	20	55.0	55.0	57.9	58.2	0.0	10.2	0.381189	46015	46648	660-695	1.000000	83.3
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	57.8	58.2	15.5	10.2	0.488942	46015	46648	692-727	1.000000	82.3
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	20	20	55.0	50.0	57.9	57.6	0.0	0.0	0.543526	46015	46648	654-689	1.000000	82.0
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GGAATCGAACCGATGACCAT	CGTCTACCGATTTCGCCATA	20	20	50.0	50.0	57.8	57.6	15.5	0.0	0.651279	46015	46648	686-721	1.000000	81.1
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GCGATGCTCTAACCTCTGAG	CGTAGCGTCTACCGATTTCG	20	20	55.0	55.0	57.9	58.6	0.0	10.2	0.749620	46015	46648	659-694	1.000000	80.4
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	CCTCGTGTCACCAGTTCAAA	TGGATAGGATGGCCTTTACG	20	20	50.0	50.0	58.1	56.8	0.0	3.3	1.273485	47587	48224	735-741	1.000000	72.4
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	CCTCGTGTCACCAGTTCAAA	GGATAGGATGGCCTTTACGT	20	20	50.0	50.0	58.1	56.8	0.0	3.3	1.275704	47587	48224	734-740	1.000000	72.4
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGATGGCC	20	21	50.0	47.6	58.1	57.7	0.0	0.0	1.349030	47587	48224	742-748	1.000000	71.8
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	CCTCGTGTCACCAGTTCAAA	TTGGATAGGATGGCCTTTACG	20	21	50.0	47.6	58.1	57.5	0.0	3.3	1.537359	47587	48224	736-742	1.000000	70.3
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	CCTCGTGTCACCAGTTCAAA	TGGATAGGATGGCCTTTACGT	20	21	50.0	47.6	58.1	58.6	0.0	3.3	1.647337	47587	48224	735-741	1.000000	69.4
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	TCATATTCGTAAAGCAGAAACA	CCGAGAAAGTCTACGGTTCG	22	20	31.8	55.0	53.9	58.1	0.0	0.0	6.230058	49897	51080	1243-1247	1.000000	42.3
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	ATCATATTCGTAAAGCAGAAACA	CCGAGAAAGTCTACGGTTCG	23	20	30.4	55.0	54.5	58.1	0.0	0.0	6.643914	49897	51080	1244-1248	1.000000	42.3
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	TCATATTCGTAAAGCAGAAACAT	CCGAGAAAGTCTACGGTTCG	23	20	30.4	55.0	54.5	58.1	0.0	0.0	6.643914	49897	51080	1243-1247	1.000000	42.3
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	TCATATTCGTAAAGCAGAAACA	GTTCGAGTCCGTATAGCCCT	22	20	31.8	55.0	53.9	58.7	0.0	0.0	6.805875	49897	51080	1228-1232	1.000000	42.3
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	CATATTCGTAAAGCAGAAACA	CCGAGAAAGTCTACGGTTCG	21	20	33.3	55.0	52.3	58.1	0.0	0.0	6.808247	49897	51080	1242-1246	1.000000	42.3
atpB-rbcL_p1	atpB-rbcL	1	CTGGCGGAAAAGCTACATCT	CACCAGCTTTGAATCCAACAC	20	21	50.0	47.6	58.0	58.0	0.0	0.0	1.047103	54088	54859	933-943	1.000000	73.1
atpB-rbcL_p2	atpB-rbcL	2	CTGGCGGAAAAGCTACATCT	ACCAGCTTTGAATCCAACACT	20	21	50.0	42.9	58.0	58.1	0.0	0.0	1.080391	54088	54859	932-942	1.000000	72.8
atpB-rbcL_p3	atpB-rbcL	3	CGCCCCAGTTTCTTTTTGTC	CACCAGCTTTGAATCCAACAC	20	21	50.0	47.6	57.9	58.0	0.0	0.0	1.151466	54088	54859	886-896	1.000000	72.3
atpB-rbcL_p4	atpB-rbcL	4	CGCCCCAGTTTCTTTTTGTC	ACCAGCTTTGAATCCAACACT	20	21	50.0	42.9	57.9	58.1	0.0	0.0	1.184754	54088	54859	885-895	1.000000	72.1
atpB-rbcL_p5	atpB-rbcL	5	TTCTTTTTGTCAAGCGTGGC	CACCAGCTTTGAATCCAACAC	20	21	45.0	47.6	57.8	58.0	0.0	0.0	1.219560	54088	54859	877-887	1.000000	71.8
petA-psbJ_p1	petA-psbJ	1	ACGTGTACAAGGCCTTTTGT	ATTCCGGATTGGGTTCATCC	20	20	45.0	50.0	58.0	57.6	17.6	4.1	0.411974	62800	63586	925-928	1.000000	79.2
petA-psbJ_p2	petA-psbJ	2	ACGTGTACAAGGCCTTTTGT	TGTGATCGGTTTAGTAGGCG	20	20	45.0	50.0	58.0	57.4	17.6	0.0	0.619192	62800	63586	963-966	1.000000	77.5
petA-psbJ_p3	petA-psbJ	3	ACGTGTACAAGGCCTTTTGT	GTGATCGGTTTAGTAGGCGT	20	20	45.0	50.0	58.0	57.4	17.6	0.0	0.620943	62800	63586	962-965	1.000000	77.4
petA-psbJ_p4	petA-psbJ	4	TCCATTACGTGTACAAGGCC	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	57.6	57.6	13.5	4.1	0.754934	62800	63586	931-934	1.000000	76.4
petA-psbJ_p5	petA-psbJ	5	CCATTACGTGTACAAGGCCT	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	57.6	57.6	13.5	4.1	0.761562	62800	63586	930-933	1.000000	76.4
petD-rpoA_p1	petD-rpoA	1	GTTTCAGTACCTGCGGGATT	AACACACGGTTCCTTTACCC	20	20	50.0	50.0	57.9	58.0	21.4	0.0	0.137944	77503	77699	664-664	1.000000	81.1
petD-rpoA_p2	petD-rpoA	2	CTCGAGCCGGATGATGAAAA	AACACACGGTTCCTTTACCC	20	20	50.0	50.0	58.1	58.0	5.0	0.0	0.088545	77503	77699	1035-1035	1.000000	80.8
petD-rpoA_p3	petD-rpoA	3	CTCGAGCCGGATGATGAAAA	TGAAGCGTCCATTTGTGTCA	20	20	50.0	45.0	58.1	58.0	5.0	0.0	0.099609	77503	77699	1445-1445	1.000000	80.6
petD-rpoA_p4	petD-rpoA	4	CTCGAGCCGGATGATGAAAA	ATTGCCTCAAACGGTCCAAT	20	20	50.0	45.0	58.1	58.1	5.0	0.0	0.136670	77503	77699	909-909	1.000000	80.5
petD-rpoA_p5	petD-rpoA	5	GTTTCAGTACCTGCGGGATT	TGAAGCGTCCATTTGTGTCA	20	20	50.0	45.0	57.9	58.0	21.4	0.0	0.149008	77503	77699	1074-1074	1.000000	80.5
rpl16-rps3_p1	rpl16-rps3	1	AAATAAAGTTTTCGCGGGCG	AGTAATGGGGGAACATGGGA	20	20	45.0	50.0	58.0	58.0	6.1	0.0	0.033810	82443	82591	1489-1497	1.000000	81.6
rpl16-rps3_p2	rpl16-rps3	2	AAATAAAGTTTTCGCGGGCG	GTCGAGGGAATTGCACGTAT	20	20	45.0	50.0	58.0	58.1	6.1	13.2	0.092276	82443	82591	1307-1315	1.000000	81.3
rpl16-rps3_p3	rpl16-rps3	3	GGTTCCTTCGTTCCCATCTC	AGTAATGGGGGAACATGGGA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.031190	82443	82591	1333-1333	0.500000	59.8
rpl16-rps3_p4	rpl16-rps3	4	GGTTCCTTCGTTCCCATCTC	GTCGAGGGAATTGCACGTAT	20	20	55.0	50.0	58.0	58.1	0.0	13.2	0.089656	82443	82591	1151-1151	0.500000	59.3
rpl16-rps3_p5	rpl16-rps3	5	GGTTCCTTCGTTCCCATCTC	GTAATGGGGGAACATGGGAC	20	20	55.0	55.0	58.0	57.9	0.0	0.0	0.092954	82443	82591	1332-1332	0.500000	59.3
ndhF-rpl32_p1	ndhF-rpl32	1	CTGGAAGTGGAAGGAAAGGT	CCGCCCAATATCCTTTCCTT	20	20	50.0	50.0	57.0	57.6	0.0	0.0	1.324438	111338	112450	1227-1247	1.000000	73.4
ndhF-rpl32_p2	ndhF-rpl32	2	AAAGAAGAAGTCCCAACCCT	CCGCCCAATATCCTTTCCTT	20	20	45.0	50.0	56.2	57.6	0.0	0.0	2.118084	111338	112450	1260-1280	1.000000	67.0
ndhF-rpl32_p3	ndhF-rpl32	3	CTGGAAGTGGAAGGAAAGGT	CCGCCCAATATCCTTTCCTTT	20	21	50.0	47.6	57.0	58.3	0.0	0.0	2.293148	111338	112450	1227-1247	1.000000	65.7
ndhF-rpl32_p4	ndhF-rpl32	4	AAAAGAAGAAGTCCCAACCCT	CCGCCCAATATCCTTTCCTT	21	20	42.9	50.0	57.0	57.6	0.0	0.0	2.338445	111338	112450	1261-1281	1.000000	65.2
ndhF-rpl32_p5	ndhF-rpl32	5	CTGGAAGTGGAAGGAAAGGTA	CCGCCCAATATCCTTTCCTT	21	20	47.6	50.0	56.9	57.6	0.0	0.0	2.434852	111338	112450	1227-1247	1.000000	64.5
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GGGAAATCTCTTTCTACCGGG	AGCGTGTCTACCGATTTCAC	21	20	52.4	50.0	57.8	58.0	6.3	0.0	1.203301	112616	113540	975-1017	1.000000	76.7
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GGGAAATCTCTTTCTACCGGG	CAGCGTGTCTACCGATTTCA	21	20	52.4	50.0	57.8	58.0	6.3	0.0	1.204672	112616	113540	976-1018	1.000000	76.7
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GGGAAATCTCTTTCTACCGGG	TCCTAAGAGCAGCGTGTCTA	21	20	52.4	50.0	57.8	57.9	6.3	0.0	1.319748	112616	113540	985-1027	1.000000	75.7
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GGGAAATCTCTTTCTACCGGG	CCTAAGAGCAGCGTGTCTAC	21	20	52.4	55.0	57.8	57.8	6.3	0.0	1.391766	112616	113540	984-1026	1.000000	75.2
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GGGAAATCTCTTTCTACCGGG	CTAAGAGCAGCGTGTCTACC	21	20	52.4	55.0	57.8	57.8	6.3	0.0	1.391766	112616	113540	983-1025	1.000000	75.2
ccsA-ndhD_p1	ccsA-ndhD	1	CGGCTCTTCTTTGTGGATCA	GTTTTTGATTCTGGACCGCG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.135394	114703	114980	937-949	1.000000	81.9
ccsA-ndhD_p2	ccsA-ndhD	2	CGGCTCTTCTTTGTGGATCA	CTCCCGGCCAACGTAATTTA	20	20	50.0	50.0	57.9	58.0	0.0	2.2	0.126145	114703	114980	1413-1425	1.000000	81.1
ccsA-ndhD_p3	ccsA-ndhD	3	TTTTTACCCAGGGCTTTGCT	CTCCCGGCCAACGTAATTTA	20	20	45.0	50.0	57.9	58.0	4.9	2.2	0.122607	114703	114980	1548-1548	0.500000	59.0
ccsA-ndhD_p4	ccsA-ndhD	4	TTTTTACCCAGGGCTTTGCT	GTTTTTGATTCTGGACCGCG	20	20	45.0	50.0	57.9	58.0	4.9	0.0	0.131857	114703	114980	1072-1072	0.500000	58.9
ccsA-ndhD_p5	ccsA-ndhD	5	TTTTTACCCAGGGCTTTGCT	GCTTGCATTACCGGGTATGA	20	20	45.0	50.0	57.9	58.0	4.9	18.9	0.133811	114703	114980	1277-1277	0.500000	58.9
ndhD-psaC_p1	ndhD-psaC	1	AACCAAGGAAATTTGTTCGT	CGCAGCATGGGTATAGCTTA	20	20	35.0	50.0	53.9	57.5	0.0	0.0	4.561815	116484	116597	158-159	1.000000	48.6
ndhD-psaC_p2	ndhD-psaC	2	TGTTAACCAAGGAAATTTGTTCGT	CGCAGCATGGGTATAGCTTA	24	20	33.3	50.0	57.8	57.5	2.5	0.0	4.665960	116484	116597	162-163	1.000000	47.6
ndhD-psaC_p3	ndhD-psaC	3	AACCAAGGAAATTTGTTCGT	CGCAGCATGGGTATAGCTTAT	20	21	35.0	47.6	53.9	58.0	0.0	0.0	5.128228	116484	116597	158-159	1.000000	45.0
ndhD-psaC_p4	ndhD-psaC	4	TGTTAACCAAGGAAATTTGTTCGT	CGCAGCATGGGTATAGCTTAT	24	21	33.3	47.6	57.8	58.0	2.5	0.0	5.232373	116484	116597	162-163	1.000000	44.9
ndhD-psaC_p5	ndhD-psaC	5	TGTTAACCAAGGAAATTTGTTCG	CGCAGCATGGGTATAGCTTA	23	20	34.8	50.0	56.2	57.5	2.5	0.0	5.250559	116484	116597	162-163	1.000000	44.9
ycf1_p1	ycf1	1	AAATTCTCCTCTTTATTTGGTAAT	CATCGTCTAGATTAATCCCCC	24	21	25.0	47.6	52.4	55.1	0.0	11.9	13.590433	124508	124627	169-172	1.000000	46.6
ycf1_p2	ycf1	2	AAATTCTCCTCTTTATTTGGTAAT	ACATCGTCTAGATTAATCCCCC	24	22	25.0	45.5	52.4	56.8	0.0	11.9	12.809457	124508	124627	170-173	1.000000	46.5
ycf1_p3	ycf1	3	TTTAAATTCTCCTCTTTATTTGGT	CATCGTCTAGATTAATCCCCC	24	21	25.0	47.6	52.6	55.1	0.0	11.9	13.379519	124508	124627	172-175	1.000000	46.5
ycf1_p4	ycf1	4	TTTAAATTCTCCTCTTTATTTGGT	ACATCGTCTAGATTAATCCCCC	24	22	25.0	45.5	52.6	56.8	0.0	11.9	12.598543	124508	124627	173-176	1.000000	46.4
ycf1_p5	ycf1	5	TTTAAATTCTCCTCTTTATTTGGT	TACATCGTCTAGATTAATCCCCC	24	23	25.0	43.5	52.6	56.7	0.0	11.9	13.690957	124508	124627	174-177	1.000000	46.4