Hotspot

ycf1

SSC · 123884-129184 · 5301 bp

Back to Cremolobus

Pi 0.0249

MarkerSeek score 56.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5301
variable_sites: 132
pi: 0.0249
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACAAACGGGCAGGTTCAATA	AAAATCGGCAGGTCAAGGAG	45.0	50.0	57.7	58.2	407 bp	1.0	80.5
ycf1_p2	ACAAACGGGCAGGTTCAATA	AACGAAACCGTGGGTCATAC	45.0	50.0	57.7	58.2	560 bp	1.0	79.9
ycf1_p3	ACAAACGGGCAGGTTCAATA	GAAACCGTGGGTCATACCAA	45.0	50.0	57.7	57.8	557 bp	1.0	79.8
ycf1_p4	ACAAACGGGCAGGTTCAATA	AAAAATCGGCAGGTCAAGGA	45.0	45.0	57.7	57.7	408 bp	1.0	79.6
ycf1_p5	ACAAACGGGCAGGTTCAATA	GGGTATAGGGGAAATTCGGC	45.0	55.0	57.7	57.8	854 bp	1.0	79.5