Hotspot

ndhG-ndhI

SSC · 118934-119266 · 333 bp

Back to Cremolobus

Pi 0.037

MarkerSeek score 62.30

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 333
variable_sites: 12
pi: 0.037
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	GTGCAGCAGCTACAAAATGG	TCAACAAACCCTACGAGCTG	50.0	50.0	58.0	57.8	986-1005 bp	1.0	80.8
ndhG-ndhI_p2	GTGCAGCAGCTACAAAATGG	GTCAACAAACCCTACGAGCT	50.0	50.0	58.0	57.8	987-1006 bp	1.0	80.8
ndhG-ndhI_p3	GCCCCCACGTAAATAAGGAG	TCAACAAACCCTACGAGCTG	55.0	50.0	58.0	57.8	1008-1027 bp	1.0	80.6
ndhG-ndhI_p4	GCCCCCACGTAAATAAGGAG	GTCAACAAACCCTACGAGCT	55.0	50.0	58.0	57.8	1009-1028 bp	1.0	80.6
ndhG-ndhI_p5	CATTTATGGCCCCCACGTAA	TCAACAAACCCTACGAGCTG	50.0	50.0	57.9	57.8	1016-1035 bp	1.0	80.4