Hotspot

petD-rpoA

LSC · 77286-77484 · 199 bp

Back to Cremolobus

Pi 0.0553

MarkerSeek score 76.40

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 199
variable_sites: 11
pi: 0.0553
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	GCCCTGTGGTTAGGTATTGG	TGGACCTTTTGAATAACAGTCA	55.0	36.4	57.9	55.6	363-364 bp	1.0	46.5
petD-rpoA_p2	GGCCCTGTGGTTAGGTATTG	TGGACCTTTTGAATAACAGTCA	55.0	36.4	57.9	55.6	364-365 bp	1.0	46.5
petD-rpoA_p3	CCGTCTTTTTGATTGGCACC	TGGACCTTTTGAATAACAGTCA	50.0	36.4	57.9	55.6	389-390 bp	1.0	46.3
petD-rpoA_p4	CTTTTTGATTGGCACCGTGG	TGGACCTTTTGAATAACAGTCA	50.0	36.4	58.2	55.6	385-386 bp	1.0	45.1
petD-rpoA_p5	CAGTAGCGACAACCGTCTTT	TGGACCTTTTGAATAACAGTCA	50.0	36.4	58.2	55.6	401-402 bp	1.0	45.0