Hotspot

psaJ-rpl33

LSC · 66538-66963 · 426 bp

Back to Cremolobus

Pi 0.0259

MarkerSeek score 70.20

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 426
variable_sites: 11
pi: 0.0259
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TTCGGTTCGTTAGCAGGTTT	AACATCTTTACCCTTGGCCA	45.0	45.0	57.8	57.0	528-532 bp	1.0	71.9
psaJ-rpl33_p2	ATGGTTCGGTTCGTTAGCAG	AACATCTTTACCCTTGGCCA	50.0	45.0	58.3	57.0	532-536 bp	1.0	71.6
psaJ-rpl33_p3	TTCGGTTCGTTAGCAGGTTT	GAACATCTTTACCCTTGGCCA	45.0	47.6	57.8	58.2	529-533 bp	1.0	70.3
psaJ-rpl33_p4	TTCGGTTCGTTAGCAGGTTT	CGAACATCTTTACCCTTGGC	45.0	50.0	57.8	56.8	530-534 bp	1.0	70.2
psaJ-rpl33_p5	TTCGGTTCGTTAGCAGGTTT	ACATCTTTACCCTTGGCCAT	45.0	45.0	57.8	56.8	527-531 bp	1.0	70.1