primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-psbA_p1	trnH-psbA	1	CTGCCTTAATCCACTTGGCT	GCTGTTGAGGCTCCATCTAC	20	20	50.0	55.0	57.9	58.1	0.0	12.9	0.188044	77	397	378-379	1.000000	82.3
trnH-psbA_p2	trnH-psbA	2	CTGCCTTAATCCACTTGGCT	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.200688	77	397	393-394	1.000000	82.1
trnH-psbA_p3	trnH-psbA	3	ACTGCCTTAATCCACTTGGC	GCTGTTGAGGCTCCATCTAC	20	20	50.0	55.0	58.2	58.1	0.0	12.9	0.213831	77	397	379-380	1.000000	82.1
trnH-psbA_p4	trnH-psbA	4	CTGCCTTAATCCACTTGGCT	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.209518	77	397	396-397	1.000000	82.0
trnH-psbA_p5	trnH-psbA	5	ACTGCCTTAATCCACTTGGC	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	58.2	57.9	0.0	13.4	0.226475	77	397	394-395	1.000000	81.8
rps16-trnQ_p1	rps16-trnQ	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6256	6694	512-514	1.000000	80.6
rps16-trnQ_p2	rps16-trnQ	2	CCTTCAATTCAAGTCGCACG	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.8	58.1	0.0	16.9	0.308340	6256	6694	522-524	1.000000	79.8
rps16-trnQ_p3	rps16-trnQ	3	CCACAGCAGATCATGTCCTT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.6	58.1	0.0	16.9	0.491095	6256	6694	538-540	1.000000	78.3
rps16-trnQ_p4	rps16-trnQ	4	TCCACAGCAGATCATGTCCT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.4	58.1	0.0	16.9	0.499524	6256	6694	539-541	1.000000	78.2
rps16-trnQ_p5	rps16-trnQ	5	ATCCACAGCAGATCATGTCC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.4	58.1	0.0	16.9	0.710916	6256	6694	540-542	1.000000	76.5
psbI-trnS_p1	psbI-trnS	1	AATGATCCCGGACGTAATCC	TTAATCGTACCGAGGGTTCG	20	20	50.0	50.0	57.2	57.2	0.7	7.4	1.683512	7828	7918	163-163	1.000000	68.5
psbI-trnS_p2	psbI-trnS	2	AATGATCCCGGACGTAATCC	GGGTTCGAATCCCTCTCTTT	20	20	50.0	50.0	57.2	56.9	0.7	17.2	1.916186	7828	7918	150-150	1.000000	66.8
psbI-trnS_p3	psbI-trnS	3	AATGATCCCGGACGTAATCC	GTTCGAATCCCTCTCTTTCCC	20	21	50.0	52.4	57.2	58.1	0.7	0.0	1.925997	7828	7918	148-148	1.000000	66.8
psbI-trnS_p4	psbI-trnS	4	AATGATCCCGGACGTAATCC	GGGTTCGAATCCCTCTCTTTC	20	21	50.0	52.4	57.2	58.1	0.7	17.2	1.925997	7828	7918	150-150	1.000000	66.7
psbI-trnS_p5	psbI-trnS	5	AATGATCCCGGACGTAATCC	GGTTCGAATCCCTCTCTTTCC	20	21	50.0	52.4	57.2	58.1	0.7	0.0	1.925997	7828	7918	149-149	1.000000	66.7
trnS-trnG_p1	trnS-trnG	1	AGTCCACTCAGCCATCTCTC	AATCAAACCGAGAGACCCTT	20	20	55.0	45.0	58.5	56.5	0.0	0.0	2.065039	8007	8654	743-746	1.000000	66.8
trnS-trnG_p2	trnS-trnG	2	AGTCCACTCAGCCATCTCTC	ATCAAACCGAGAGACCCTTT	20	20	55.0	45.0	58.5	56.5	0.0	0.0	2.065039	8007	8654	742-745	1.000000	66.8
trnS-trnG_p3	trnS-trnG	3	AGTCCACTCAGCCATCTCTC	AATCAAACCGAGAGACCCTTT	20	21	55.0	42.9	58.5	57.2	0.0	0.0	2.306051	8007	8654	743-746	1.000000	64.9
trnS-trnG_p4	trnS-trnG	4	TAGTCCACTCAGCCATCTCT	AATCAAACCGAGAGACCCTT	20	20	50.0	45.0	57.2	56.5	0.0	0.0	2.385713	8007	8654	744-747	1.000000	64.2
trnS-trnG_p5	trnS-trnG	5	TAGTCCACTCAGCCATCTCT	ATCAAACCGAGAGACCCTTT	20	20	50.0	45.0	57.2	56.5	0.0	0.0	2.385713	8007	8654	743-746	1.000000	64.2
trnR-atpA_p1	trnR-atpA	1	GCGAAAAGCGTCCATTGTC	TCAAGAGCAACTGGAACGTT	19	20	52.6	45.0	58.0	57.7	0.0	0.0	1.327159	9701	9995	417-422	1.000000	72.2
trnR-atpA_p2	trnR-atpA	2	GCGAAAAGCGTCCATTGTC	TTCAAGAGCAACTGGAACGT	19	20	52.6	45.0	58.0	57.7	0.0	0.0	1.327159	9701	9995	418-423	1.000000	72.2
trnR-atpA_p3	trnR-atpA	3	GCGAAAAGCGTCCATTGTC	ACATTAACCGCTGAAGCAGA	19	20	52.6	45.0	58.0	57.5	0.0	2.8	1.474271	9701	9995	458-463	1.000000	70.8
trnR-atpA_p4	trnR-atpA	4	GCGAAAAGCGTCCATTGTC	AGACATTAACCGCTGAAGCA	19	20	52.6	45.0	58.0	57.5	0.0	2.8	1.474271	9701	9995	460-465	1.000000	70.8
trnR-atpA_p5	trnR-atpA	5	GCGAAAAGCGTCCATTGTCT	TCAAGAGCAACTGGAACGTT	20	20	50.0	45.0	59.5	57.7	0.0	0.0	1.808267	9701	9995	417-422	1.000000	68.4
atpF-atpH_p1	atpF-atpH	1	ATTAAACCCGAAACTCCCGG	TGGCCTGGTTGTAGCATTAG	20	20	50.0	50.0	57.9	57.6	0.0	3.3	0.536681	12868	13329	580-596	1.000000	77.2
atpF-atpH_p2	atpF-atpH	2	CCAGTGACCCAAGTAAACGA	TGGCCTGGTTGTAGCATTAG	20	20	50.0	50.0	57.5	57.6	0.0	3.3	0.955129	12868	13329	553-569	1.000000	73.9
atpF-atpH_p3	atpF-atpH	3	ATTAAACCCGAAACTCCCGG	TTTATGGCCTGGTTGTAGCA	20	20	50.0	45.0	57.9	57.1	0.0	3.3	1.007483	12868	13329	584-600	1.000000	73.4
atpF-atpH_p4	atpF-atpH	4	ATTAAACCCGAAACTCCCGG	GGCCTGGTTGTAGCATTAGC	20	20	50.0	55.0	57.9	59.0	0.0	0.0	1.093272	12868	13329	579-595	1.000000	72.8
atpF-atpH_p5	atpF-atpH	5	GGCCAGTGACCCAAGTAAAC	TGGCCTGGTTGTAGCATTAG	20	20	55.0	50.0	58.8	57.6	0.0	3.3	1.173552	12868	13329	555-571	1.000000	72.2
atpH-atpI_p1	atpH-atpI	1	AACCGAAGCAGCAGAAATCA	TACAAGCGGGATTCAAGCTC	20	20	45.0	50.0	58.1	58.0	0.0	5.7	0.127956	13576	14068	599-602	1.000000	81.9
atpH-atpI_p2	atpH-atpI	2	TACCTTGACCAACTCCAGGT	TACAAGCGGGATTCAAGCTC	20	20	50.0	50.0	57.9	58.0	0.0	5.7	0.136455	13576	14068	657-660	1.000000	81.6
atpH-atpI_p3	atpH-atpI	3	GTCCAATAGAAGCAAGCCCA	TACAAGCGGGATTCAAGCTC	20	20	50.0	50.0	57.9	58.0	0.0	5.7	0.152946	13576	14068	639-642	1.000000	81.5
atpH-atpI_p4	atpH-atpI	4	AACCGAAGCAGCAGAAATCA	TTTTTGCCACTTTAGCTGCG	20	20	45.0	45.0	58.1	57.9	0.0	0.0	0.236520	13576	14068	576-579	1.000000	81.2
atpH-atpI_p5	atpH-atpI	5	GCTGTACCTTGACCAACTCC	TACAAGCGGGATTCAAGCTC	20	20	55.0	50.0	58.2	58.0	0.0	5.7	0.214492	13576	14068	661-664	1.000000	80.9
rpoB-trnC_p1	rpoB-trnC	1	GTTCCCTCTTTTCCATCCCC	CCGGATTTGAACTGGGGAAA	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.227525	26388	27482	1183-1200	1.000000	80.5
rpoB-trnC_p2	rpoB-trnC	2	GTTCCCTCTTTTCCATCCCC	AAAAGGATTTGCAGTCCCCC	20	20	55.0	50.0	57.9	58.4	0.0	14.6	0.500888	26388	27482	1165-1182	1.000000	78.4
rpoB-trnC_p3	rpoB-trnC	3	GTTCCCTCTTTTCCATCCCC	TCAAGTCTGTTGTTGAGGCG	20	20	55.0	50.0	57.9	58.4	0.0	0.0	0.563463	26388	27482	1207-1224	1.000000	77.8
rpoB-trnC_p4	rpoB-trnC	4	TGTTCCCTCTTTTCCATCCC	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	57.1	58.1	0.0	0.0	0.981431	26388	27482	1184-1201	1.000000	74.5
rpoB-trnC_p5	rpoB-trnC	5	GTTCCCTCTTTTCCATCCCC	AAGTCTGTTGTTGAGGCGG	20	19	55.0	52.6	57.9	58.0	0.0	0.0	1.152069	26388	27482	1205-1222	1.000000	73.1
trnE-trnT_p1	trnE-trnT	1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	57.8	58.0	0.0	0.0	0.165150	30653	31473	959-962	1.000000	81.0
trnE-trnT_p2	trnE-trnT	2	TTTCGTAGTACCCTACCCCC	CCATGGCGTTACTCTACCAC	20	20	55.0	55.0	57.8	58.1	0.0	0.0	0.223247	30653	31473	938-941	1.000000	80.6
trnE-trnT_p3	trnE-trnT	3	TTTCGTAGTACCCTACCCCC	TGGCGTTACTCTACCACTGA	20	20	55.0	50.0	57.8	58.1	0.0	0.0	0.245401	30653	31473	935-938	1.000000	80.4
trnE-trnT_p4	trnE-trnT	4	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	58.3	58.0	0.0	0.0	0.269359	30653	31473	921-924	1.000000	80.3
trnE-trnT_p5	trnE-trnT	5	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.287509	30653	31473	900-903	1.000000	80.2
psbC-trnS_p1	psbC-trnS	1	TCGTGGGTCATTTATGGCAC	GATGGCTCCGGTCTTGAAAA	20	20	50.0	50.0	58.3	58.2	0.0	0.0	0.433551	35204	35384	363-364	1.000000	78.5
psbC-trnS_p2	psbC-trnS	2	TCGTGGGTCATTTATGGCAC	GGGTTCGAATCCCTCTCTCT	20	20	50.0	55.0	58.3	58.2	0.0	17.2	0.471697	35204	35384	313-314	1.000000	78.5
psbC-trnS_p3	psbC-trnS	3	CGGCAGCAGGATTTGAAAAA	GGGTTCGAATCCCTCTCTCT	20	20	45.0	55.0	57.6	58.2	0.0	17.2	0.654128	35204	35384	274-275	1.000000	77.4
psbC-trnS_p4	psbC-trnS	4	CGGCAGCAGGATTTGAAAAA	GATGGCTCCGGTCTTGAAAA	20	20	45.0	50.0	57.6	58.2	0.0	0.0	0.615983	35204	35384	324-325	1.000000	77.3
psbC-trnS_p5	psbC-trnS	5	TTCGTGGGTCATTTATGGCA	GATGGCTCCGGTCTTGAAAA	20	20	45.0	50.0	57.5	58.2	0.0	0.0	0.677428	35204	35384	364-365	1.000000	76.6
trnT-trnL_p1	trnT-trnL	1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCAATTCC	20	20	55.0	55.0	57.9	58.2	0.0	10.2	0.283643	46290	47104	876-891	1.000000	79.5
trnT-trnL_p2	trnT-trnL	2	TATGGAAAAGCCGGCTATCG	CCGTAGCGTCTACCAATTCC	20	20	50.0	55.0	57.8	58.2	33.1	10.2	0.314721	46290	47104	927-942	1.000000	79.2
trnT-trnL_p3	trnT-trnL	3	GCGATGCTCTAACCTCTGAG	AGTCCGTAGCGTCTACCAAT	20	20	55.0	50.0	57.9	58.2	0.0	10.2	0.372548	46290	47104	879-894	1.000000	78.8
trnT-trnL_p4	trnT-trnL	4	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCAATTCC	20	20	50.0	55.0	57.8	58.2	15.5	10.2	0.391397	46290	47104	908-923	1.000000	78.6
trnT-trnL_p5	trnT-trnL	5	TATGGAAAAGCCGGCTATCG	AGTCCGTAGCGTCTACCAAT	20	20	50.0	50.0	57.8	58.2	33.1	10.2	0.403626	46290	47104	930-945	1.000000	78.5
ndhJ-psbG_p1	ndhJ-psbG	1	CCTTGGTAATCGAAGCCCAA	AGTCCAGTATCTTCCCACGA	20	20	50.0	50.0	57.9	57.5	11.2	0.0	0.649671	48990	49092	209-210	1.000000	76.7
ndhJ-psbG_p2	ndhJ-psbG	2	GCCCAACGATCTATGAACCA	AGTCCAGTATCTTCCCACGA	20	20	50.0	50.0	57.7	57.5	0.0	0.0	0.852471	48990	49092	195-196	1.000000	75.2
ndhJ-psbG_p3	ndhJ-psbG	3	TTCCTTGGTAATCGAAGCCC	AGTCCAGTATCTTCCCACGA	20	20	50.0	50.0	57.6	57.5	0.0	0.0	0.940197	48990	49092	211-212	1.000000	74.4
ndhJ-psbG_p4	ndhJ-psbG	4	CGAAGCCCAACGATCTATGA	AGTCCAGTATCTTCCCACGA	20	20	50.0	50.0	57.5	57.5	0.0	0.0	1.035940	48990	49092	199-200	1.000000	73.7
ndhJ-psbG_p5	ndhJ-psbG	5	TCGAAGCCCAACGATCTATG	AGTCCAGTATCTTCCCACGA	20	20	50.0	50.0	57.5	57.5	0.0	0.0	1.035940	48990	49092	200-201	1.000000	73.7
psaJ-rpl33_p1	psaJ-rpl33	1	TTCGGTTCGTTAGCAGGTTT	AACATCTTTACCCTTGGCCA	20	20	45.0	45.0	57.8	57.0	0.0	0.0	1.234342	66538	66963	528-532	1.000000	71.9
psaJ-rpl33_p2	psaJ-rpl33	2	ATGGTTCGGTTCGTTAGCAG	AACATCTTTACCCTTGGCCA	20	20	50.0	45.0	58.3	57.0	0.0	0.0	1.268728	66538	66963	532-536	1.000000	71.6
psaJ-rpl33_p3	psaJ-rpl33	3	TTCGGTTCGTTAGCAGGTTT	GAACATCTTTACCCTTGGCCA	20	21	45.0	47.6	57.8	58.2	0.0	0.0	1.440883	66538	66963	529-533	1.000000	70.3
psaJ-rpl33_p4	psaJ-rpl33	4	TTCGGTTCGTTAGCAGGTTT	CGAACATCTTTACCCTTGGC	20	20	45.0	50.0	57.8	56.8	0.0	0.0	1.448554	66538	66963	530-534	1.000000	70.2
psaJ-rpl33_p5	psaJ-rpl33	5	TTCGGTTCGTTAGCAGGTTT	ACATCTTTACCCTTGGCCAT	20	20	45.0	45.0	57.8	56.8	0.0	6.1	1.469049	66538	66963	527-531	1.000000	70.1
petD-rpoA_p1	petD-rpoA	1	GCCCTGTGGTTAGGTATTGG	TGGACCTTTTGAATAACAGTCA	20	22	55.0	36.4	57.9	55.6	0.0	0.0	4.484754	77286	77484	363-364	1.000000	46.5
petD-rpoA_p2	petD-rpoA	2	GGCCCTGTGGTTAGGTATTG	TGGACCTTTTGAATAACAGTCA	20	22	55.0	36.4	57.9	55.6	0.0	0.0	4.484754	77286	77484	364-365	1.000000	46.5
petD-rpoA_p3	petD-rpoA	3	CCGTCTTTTTGATTGGCACC	TGGACCTTTTGAATAACAGTCA	20	22	50.0	36.4	57.9	55.6	0.0	0.0	4.496545	77286	77484	389-390	1.000000	46.3
petD-rpoA_p4	petD-rpoA	4	CTTTTTGATTGGCACCGTGG	TGGACCTTTTGAATAACAGTCA	20	22	50.0	36.4	58.2	55.6	0.0	0.0	4.649928	77286	77484	385-386	1.000000	45.1
petD-rpoA_p5	petD-rpoA	5	CAGTAGCGACAACCGTCTTT	TGGACCTTTTGAATAACAGTCA	20	22	50.0	36.4	58.2	55.6	0.0	0.0	4.652402	77286	77484	401-402	1.000000	45.0
rpl36-rps8_p1	rpl36-rps8	1	GTTTATGCCTCGGGTTGGAA	TCGGCGGAGAAATTTTGTGT	20	20	50.0	45.0	58.2	58.4	0.0	7.2	0.570997	79187	79669	609-615	1.000000	77.7
rpl36-rps8_p2	rpl36-rps8	2	TTTATGCCTCGGGTTGGAAC	TCGGCGGAGAAATTTTGTGT	20	20	50.0	45.0	58.2	58.4	0.0	7.2	0.570997	79187	79669	608-614	1.000000	77.7
rpl36-rps8_p3	rpl36-rps8	3	GTTTATGCCTCGGGTTGGAA	CGGCGGAGAAATTTTGTGTT	20	20	50.0	45.0	58.2	57.6	0.0	0.0	0.586718	79187	79669	608-614	1.000000	77.5
rpl36-rps8_p4	rpl36-rps8	4	TTTATGCCTCGGGTTGGAAC	CGGCGGAGAAATTTTGTGTT	20	20	50.0	45.0	58.2	57.6	0.0	0.0	0.586718	79187	79669	607-613	1.000000	77.5
rpl36-rps8_p5	rpl36-rps8	5	TTTATGCCTCGGGTTGGAAC	TCCACGAATTTTAGGCGGAA	20	20	50.0	45.0	58.2	57.5	0.0	0.0	0.647147	79187	79669	694-700	1.000000	76.8
rpl32-trnL_p1	rpl32-trnL	1	AACGAGCACTCTAGTACGGA	TGCAGACTGAGGCATTTTGT	20	20	50.0	45.0	57.9	58.0	0.0	6.7	0.119533	113415	114289	1835-1879	1.000000	80.9
rpl32-trnL_p2	rpl32-trnL	2	AACGAGCACTCTAGTACGGA	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.229283	113415	114289	1305-1341	1.000000	80.7
rpl32-trnL_p3	rpl32-trnL	3	AACGAGCACTCTAGTACGGA	TAACCACTGGGACTGGAGAG	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.166422	113415	114289	1868-1912	1.000000	80.5
rpl32-trnL_p4	rpl32-trnL	4	AACGAGCACTCTAGTACGGA	AGCAGCGTGTCTACCAATTT	20	20	50.0	45.0	57.9	57.8	0.0	0.0	0.290010	113415	114289	1298-1334	1.000000	80.2
rpl32-trnL_p5	rpl32-trnL	5	AACGAGCACTCTAGTACGGA	CTAAGAGCAGCGTGTCTACC	20	20	50.0	55.0	57.9	57.8	0.0	0.0	0.301301	113415	114289	1303-1339	1.000000	80.1
ndhG-ndhI_p1	ndhG-ndhI	1	GTGCAGCAGCTACAAAATGG	TCAACAAACCCTACGAGCTG	20	20	50.0	50.0	58.0	57.8	1.8	0.0	0.177120	118934	119266	986-1005	1.000000	80.8
ndhG-ndhI_p2	ndhG-ndhI	2	GTGCAGCAGCTACAAAATGG	GTCAACAAACCCTACGAGCT	20	20	50.0	50.0	58.0	57.8	1.8	0.0	0.178621	118934	119266	987-1006	1.000000	80.8
ndhG-ndhI_p3	ndhG-ndhI	3	GCCCCCACGTAAATAAGGAG	TCAACAAACCCTACGAGCTG	20	20	55.0	50.0	58.0	57.8	0.0	0.0	0.200646	118934	119266	1008-1027	1.000000	80.6
ndhG-ndhI_p4	ndhG-ndhI	4	GCCCCCACGTAAATAAGGAG	GTCAACAAACCCTACGAGCT	20	20	55.0	50.0	58.0	57.8	0.0	0.0	0.202147	118934	119266	1009-1028	1.000000	80.6
ndhG-ndhI_p5	ndhG-ndhI	5	CATTTATGGCCCCCACGTAA	TCAACAAACCCTACGAGCTG	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.223868	118934	119266	1016-1035	1.000000	80.4
ycf1_p1	ycf1	1	ACAAACGGGCAGGTTCAATA	AAAATCGGCAGGTCAAGGAG	20	20	45.0	50.0	57.7	58.2	0.0	0.0	0.453550	124444	124593	407-407	1.000000	80.5
ycf1_p2	ycf1	2	ACAAACGGGCAGGTTCAATA	AACGAAACCGTGGGTCATAC	20	20	45.0	50.0	57.7	58.2	0.0	0.0	0.480391	124444	124593	560-560	1.000000	79.9
ycf1_p3	ycf1	3	ACAAACGGGCAGGTTCAATA	GAAACCGTGGGTCATACCAA	20	20	45.0	50.0	57.7	57.8	0.0	0.0	0.467996	124444	124593	557-557	1.000000	79.8
ycf1_p4	ycf1	4	ACAAACGGGCAGGTTCAATA	AAAAATCGGCAGGTCAAGGA	20	20	45.0	45.0	57.7	57.7	0.0	0.0	0.566299	124444	124593	408-408	1.000000	79.6
ycf1_p5	ycf1	5	ACAAACGGGCAGGTTCAATA	GGGTATAGGGGAAATTCGGC	20	20	45.0	55.0	57.7	57.8	0.0	0.0	0.475676	124444	124593	854-854	1.000000	79.5