Hotspot

ndhA

SSC · 117940-120088 · 2149 bp

Back to Cremanthodium

Pi 0.00208

MarkerSeek score 43.20

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2149
variable_sites: 10
pi: 0.00208
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGAACTCCGTTACCATAGGGA	TCATTATGGGAGAAGTTGATCGT	47.6	39.1	57.9	57.4	2273-2282 bp	1.0	42.8
ndhA_p2	ACTCCGTTACCATAGGGAACA	TCATTATGGGAGAAGTTGATCGT	47.6	39.1	58.2	57.4	2270-2279 bp	1.0	42.3
ndhA_p3	TGAACTCCGTTACCATAGGGA	TGGGAGAAGTTGATCGTTAA	47.6	40.0	57.9	54.0	2267-2276 bp	1.0	40.6
ndhA_p4	TGAACTCCGTTACCATAGGGA	TCATTATGGGAGAAGTTGATCGTT	47.6	37.5	57.9	58.0	2273-2282 bp	1.0	40.6
ndhA_p5	TGAACTCCGTTACCATAGGGA	CATTATGGGAGAAGTTGATCGT	47.6	40.9	57.9	56.0	2272-2281 bp	1.0	40.6