Hotspot

clpP

LSC · 69131-71149 · 2019 bp

Back to Cremanthodium

Pi 0.00159

MarkerSeek score 59.10

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2019
variable_sites: 8
pi: 0.00159
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	ATTGACGCGAAATACAGGCT	AAGGCTTTGTTACGTTTCCA	45.0	40.0	58.0	55.8	2103-2113 bp	1.0	62.6
clpP_p2	CTCGGCAGGGTTAACTTCAA	AAGGCTTTGTTACGTTTCCA	50.0	40.0	57.8	55.8	2130-2140 bp	1.0	61.3
clpP_p3	AACTCGGCAGGGTTAACTTC	AAGGCTTTGTTACGTTTCCA	50.0	40.0	57.8	55.8	2132-2142 bp	1.0	61.3
clpP_p4	ATTGACGCGAAATACAGGCT	AAAGGCTTTGTTACGTTTCCA	45.0	38.1	58.0	56.6	2104-2114 bp	1.0	60.8
clpP_p5	ATTGACGCGAAATACAGGCT	AGGCTTTGTTACGTTTCCAT	45.0	40.0	58.0	55.6	2102-2112 bp	1.0	60.7