Hotspot

ndhF-rpl32

SSC · 110438-111508 · 1071 bp

Back to Cremanthodium

Pi 0.00931

MarkerSeek score 55.60

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1071
variable_sites: 21
pi: 0.00931
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTTGCCGTCGGAAAAAGAAG	TAACGCTGCCCAAAATCCTT	50.0	45.0	58.0	58.1	1045-1256 bp	1.0	84.6
ndhF-rpl32_p2	GTTGCCGTCGGAAAAAGAAG	TTAACGCTGCCCAAAATCCT	50.0	45.0	58.0	58.1	1046-1257 bp	1.0	84.6
ndhF-rpl32_p3	TGCCGTCGGAAAAAGAAGAA	TAACGCTGCCCAAAATCCTT	45.0	45.0	58.0	58.1	1043-1254 bp	1.0	84.5
ndhF-rpl32_p4	TGCCGTCGGAAAAAGAAGAA	TTAACGCTGCCCAAAATCCT	45.0	45.0	58.0	58.1	1044-1255 bp	1.0	84.5
ndhF-rpl32_p5	TTGCCGTCGGAAAAAGAAGA	TAACGCTGCCCAAAATCCTT	45.0	45.0	58.0	58.1	1044-1255 bp	1.0	84.5