Hotspot

rps8-rpl14

LSC · 79670-79872 · 203 bp

Back to Cremanthodium

Pi 0.00198

MarkerSeek score 56.10

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 203
variable_sites: 1
pi: 0.00198
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	ACTCGAACCACGCTTTTTCT	GAACTCGAGTTTTTGGTGCG	45.0	50.0	58.1	58.0	362-366 bp	1.0	80.5
rps8-rpl14_p2	ACTCGAACCACGCTTTTTCT	CGATTGCCCGAGAATTGAGA	45.0	50.0	58.1	58.1	344-348 bp	1.0	80.3
rps8-rpl14_p3	ACTCGAACCACGCTTTTTCT	TTGCCCGAGAATTGAGACAG	45.0	50.0	58.1	57.9	341-345 bp	1.0	80.0
rps8-rpl14_p4	ACTCGAACCACGCTTTTTCT	GCCCGAGAATTGAGACAGTT	45.0	50.0	58.1	57.9	339-343 bp	1.0	80.0
rps8-rpl14_p5	ACTCGAACCACGCTTTTTCT	ACTCGAGTTTTTGGTGCGAT	45.0	45.0	58.1	58.1	360-364 bp	1.0	79.7