Hotspot

atpF-atpA

LSC · 28127-28199 · 73 bp

Back to Cremanthodium

Pi 0.0164

MarkerSeek score 57.00

Primer available yes

Label atpF-atpA

Feature Metadata

label_name: atpF-atpA
feature_type: igs
region: LSC
length_bp: 73
variable_sites: 3
pi: 0.0164
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpA_p1	ATATTGGCATATTGGGGGCG	GTCGGCTTGAATGGTTACCA	50.0	50.0	58.1	58.2	134-139 bp	1.0	80.3
atpF-atpA_p2	ATATTGGCATATTGGGGGCG	ATTTCGTCGGCTTGAATGGT	50.0	45.0	58.1	58.2	139-144 bp	1.0	80.2
atpF-atpA_p3	ATATTGGCATATTGGGGGCG	CAATGCCGTCACCTACTTGA	50.0	50.0	58.1	57.9	231-236 bp	1.0	79.8
atpF-atpA_p4	ATATTGGCATATTGGGGGCG	TCGTCGGCTTGAATGGTTAC	50.0	50.0	58.1	58.3	136-141 bp	1.0	79.4
atpF-atpA_p5	ATATTGGCATATTGGGGGCG	GCTCAATACGTTCGCGGATA	50.0	50.0	58.1	58.2	168-173 bp	1.0	79.3